The first indication of allergies and assessment of their intensity is done by measuring eosinophils, which are a specific type of white immune cells. Their measurement is done with the general blood - blood chart and the microscopic examination of the blood. It is also used in monitoring – assessing the effectiveness of possible treatment.

A diagnostic test to detect and calculate the intensity of allergies is also used in monitoring - assessing of effectiveness of a possible treatment.

Once the allergy is established, we identify the natural or artificial substances that cause allergic reactions through specialized analyses, called "Rast tests". Rast tests help us to detect and quantitatively measure the existence of IgE antibodies of an allergic reaction for each substance separately. The main categories, which contain a lot of independent specialized tests, are:

1. Rast G: GRASS POLLEN (Bermuda grass, grass, Vernal grass, Stubble, Oats, Rye, Corn, etc.)
2. Rast W: POLLEN from Weed Flower Pollen (Weed, Marguerite, Chicory, Nettle, Wall Pellitory, Chamomile, Carnation, Rose, etc.)
3. Rast T: POLLEN from TREES (hazelnut, olive, walnut, sycamore, elm, willow, eucalyptus, orange tree, tonsil, fir, mulberry, etc.)
4. Rast D: MITES
5. Rast H: HOUSEDUST
6. Rast B – K – O: FABRICS (linen, wool, silk, latex, cotton)
7. Rast F : FOOD (egg, dairy, cereals, fruit, nuts, vegetables, meat, fish, etc.)
8. Rast E: ANIMAL EPITHELIUM (cat, dog, horse, cow, goat, rabbit, hamster, chicken, duck, parrot, pigeon, etc.)
9. Rast I: INSECTS (bee venom – wasp, fly, mosquito, horsefly, etc.)
10. Rast C: DRUGS (in various antibiotics penicillin, erythromycin, Amoxil, cefaclor, ciprofloxacin, etc.)

In the investigation of allergies and especially in the assessment of allergic shocks can be optionally measured the:

• Histamine
• Tryptase